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Understanding and Manipulating Protein-Protein Interactions

Module: 
3
Section: 
D

Instructor: Harold P. Erickson
Summary: Proteins are the machines of the cells. A few enzymes operate alone, but most proteins interact with others to form more complex machines. In this unit we will learn the basic principles of protein-protein interaction and bonding, and address the following questions.
How big is a protein molecule; how do you determine if it is a monomer or tetramer; how do you determine its shape? What is the structure of a protein-protein bond? How many amino acids are in contact? How does the dissociation constant relate to the strength of the bond? How fast do two proteins form a bond, and once formed how long does the complex last before it dissociates? If you want to eliminate or reduce a protein-protein bond by mutagenesis, how many amino acids to you need to change? How do you decide which ones?

Readings: 

Molecular Biology of the Cell, Alberts et al.
Chapter 3 - Proteins.
Chapter 2 (to review basic biochemistry. Most important is to know the amino acids, which ones are hydrophobic, hydrophilic, charged)