My lab uses genetics and biochemistry to investigate the transcription apparatus in eukaryotes, focusing on RNA polymerase II and factors affecting its activity. These studies contribute to understanding gene expression during development.
We are currently focusing our attention on determining physiological roles for the unique C-terminal repeat domain (CTD) of the largest RNA polymerase II subunit, which is hyperphosphorylated during transcript elongation. We are discovering novel phospho-CTD-associating proteins ("PCAPs") that have roles in pre-mRNA processing and in other RNA and DNA transactions. Recently we identified yeast and mammalian PCAPs with known or suspected epigenetic functions that are important in regulating gene expression during differentiation and development. We plan to investigate these proteins in Drosophila, because of its available advanced developmental genetic techniques.
DBP Home 