Elwood Linney, Molecular Genetics & Microbiology

This laboratory uses molecular, transgenic, genetic and computer approaches towards studying the regulation of genes during embryonic development with a particular focus on visualizing gene expression in developing zebrafish embryos and young fish. We are using DNA microinjection techniques and pseudotyped retroviral vectors to introduce new genes into the zebrafish genome. Expression of the genes is monitored with fluorescent reporter genes and the images are captured on an confocal microscope and re-constructed using computer rendering techniques. Retroviral vectors are also being used as insertional mutagens coupled with visible screening of changes in gene expression to identify and isolate genes involved in receptor signal transduction pathways.