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Michel Bagnat

Associate Professor
Cell Biology
(919) 681-9268
Research Interest: 
Developmental biology
Membranes and organelles
Research Summary: 
Cellular and physiologic mechanisms controlling morphogenesis
Research Description: 

Our laboratory is interested in studying how basic cellular processes define the shape and size of complex multicellular structures such as organs. Fluid movement into enclosed luminal or intracellular spaces creates hydrostatic pressure that can serve as a driving force for organogenesis and long range morphogenetic events such as axis elongation.

Our major goal to understand the role hydrostatic pressure plays as a developmental force. Using zebrafish we investigate:
1-Regulation of fluid secretion and the role of fluid pressure in organogenesis.
2-The biogenesis and function of fluid-filled vacuoles in the notochord during embryogenesis and spine morphogenesis.
3-Cellular mechanisms controlling epithelial polarization and lumen formation in the gut tube.

Notochord vacuoles are lysosome-related organelles that function in axis and spine morphogenesis.
Ellis K, Bagwell J, Bagnat M.
J Cell Biol. 2013. 200:667-79.

Single continuous lumen formation in the zebrafish gut is mediated by smoothened-dependent tissue remodeling.
Alvers AL, Ryan S, Scherz PJ, Huisken J, Bagnat M.
Development. 2014. 141:1110-9.

Cftr controls lumen expansion and function of Kupffer's vesicle in zebrafish.
Navis A, Marjoram L, Bagnat M.
Development. 2013. 140:1703-12.

Rapid identification of kidney cyst mutations by whole exome sequencing in zebrafish.
Ryan S, Willer J, Marjoram L, Bagwell J, Mankiewicz J, Leshchiner I, Goessling W, Bagnat M, Katsanis N.
Development. 2013. 140:4445-51.

Cse1l is a negative regulator of CFTR-dependent fluid secretion.
Bagnat M, Navis A, Herbstreith S, Brand-Arzamendi K, Curado S, Gabriel S, Mostov K, Huisken J, Stainier DY.
Curr Biol. 2010. 20:1840-5.